A Proposal for Using Modified Site-specific Recombination Systems for Making Insertions into a Chosen Chromosomal Sitein vivoBased on the Analysis of I Phage Integration

A proposal is presented here to use modified mobile genetic elements for making chromosomal insertionsin vivo, into any desired chromosomal site. The idea lies in replacing the sequences which direct specificity of integration of those elements with other DNA sequences, homologous to the desired target chromosomal site. The idea is analysed on the basis of a I phage integration system. A design for a universal system for making chromosomal insertions into any site of choice is proposed.