A quantitative kinetic model for ATP-induced intracellular oscillations

A quantitative kinetic model is proposed to simulate the ATP-induced intracellular Ca 2 + oscillations. The quantitative effect of ATP concentration upon the oscillations was successfully simulated. Our simulation results support previous experimental explanations that the Ca 2 + oscillations are mainly due to interaction of Ca 2 + release from the endoplasmic reticulum (ER) and the ATP-dependent Ca 2 + pump back into the ER, and the oscillations are prolonged by extracellular Ca 2 + entry that maintains the constant Ca 2 + supplies to its intracellular stores. The model is also able to simulate the sudden disappearance phenomenon of the Ca 2 + oscillations observed in some cell types by taking into account of the biphasic characteristic of the Ca 2 + release from the endoplasmic reticulum (ER). Moreover, the model simulation results for the Ca 2 + oscillations characteristics such as duration, peak [ Ca 2 + ] cyt , and average interval, etc., lead to prediction of some possible factors responsible for the variations of Ca 2 + oscillations in different types of cells.