The Inkjet Printing of Reducible AgNPs as Amperometric Glucose Biosensor Electrodes

The enzymes immobilization is a crucially effective factor for biosensor preparation. Metal nanoparticles are potentially able to immobilize the enzymes due to their unique properties including large surface-to-volume ratio, high surface reaction activity, high catalytic efficiency, and strong adsorption ability. A novel and highly sensitive amperometric glucose biosensor was obtained using the inkjet printing of reducing agents and metal salts. In this method, AgNPs are reduced from silver nitrate using ascorbic acid solutions on paper as substrate. Inkjet deposited patterns are utilized as electrodes in different electrochemical experiments and their morphology were investigated by SEM imaging. Results of cyclic voltammetry tests revealed that the glucose oxidase could be highly stabilized and immobilized on the surface of silver nanoparticles. Furthermore, the biosensor has a very high sensitivity of 8 µA/ mm2 and a low detection limit of about 5 mM. These results demonstrate that inkjet printing AgNPs have potential applications in glucose biosensors.