Title of article
Optimization of alkaline phosphatase gene expression in E. coli
Author/Authors
Mir Mohammad Sadeghi، H. نويسنده Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Sciences Research Center School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. , , Rabbani، M. نويسنده Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R.Iran. , , Fazeli، M. S. نويسنده ,
Issue Information
دوفصلنامه با شماره پیاپی 0 سال 2008
Pages
5
From page
35
To page
39
Abstract
Alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. In the present study, alkaline phosphatase gene was inserted into the pET15b vector. The recombinant DNA was then expressed using IPTG as an inducer. The expression of this enzyme was optimized by changing expression conditions such as IPTG concentration, time, and temperature. The obtained results demonstrate that with the expression system used, large quantities of active alkaline phosphatase can be produced in E. coli.
Journal title
Research in Pharmaceutical Sciences
Serial Year
2008
Journal title
Research in Pharmaceutical Sciences
Record number
1588151
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