Author/Authors :
Evans، نويسنده , , Neil D. and Errington، نويسنده , , Rachel J. and Shelley، نويسنده , , Michael and Feeney، نويسنده , , Graham P. and Chapman، نويسنده , , Michael J. and Godfrey، نويسنده , , Keith R. and Smith، نويسنده , , Paul J. and Chappell، نويسنده , , Michael J.، نويسنده ,
Abstract :
In this paper a compartmental modelling approach is applied to provide a mathematical description of the activity of the anti-cancer agent topotecan, and delivery to its nuclear DNA target following administration. The activity of topotecan in defined buffers is first modelled using a linear two compartment model that then forms the basis of a cell based model for drug activity in live cell experiments. An identifiability analysis is performed before parameter estimation to ensure that the model output (i.e., continuous, perfect and noise-free data) uniquely determines the parameters. Parameter estimation is performed using experimental data which offers concentrations of active and inactive forms of topotecan from high performance liquid chromatography methods.
Keywords :
compartmental models , Topotecan , Drug kinetics , identifiability
