Title of article
Immune efficacy of OmpH and OmpA DNA vaccines against avian Pasteurella multocida
Author/Authors
گونگ، كوايانگ نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Gong,, Q , كوئين، كوئيلي نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Qin, C. L , نيو، مينگفو نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Niu, M. F. , چنگ، مينگ نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Cheng, M , سون، زيااوفي نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Sun, X. F , ژانگ، آييگواو نويسنده Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Zhang, A. G
Issue Information
فصلنامه با شماره پیاپی 44 سال 2013
Pages
6
From page
197
To page
202
Abstract
پاستورولا مولتوسيدا در طيور عامل وباي ماكيان است. اثر محافظتي به دست آمده از طريق واكسنهاي معمول رضايتبخش نميباشد، لذا تحقيقات بر روي واكسنهاي جديد بر عليه پاستورولا مولتوسيدا در طيور ضروري به نظر ميرسد. در اين مطالعه ژنهاي OmpH و OmpA رمزدهي كننده پروتئينهاي H و A غشاء بيروني پاستورولا مولتوسيدا به درون ناقل يوكاريوت pcDNA3.1(+) بيان كننده كلون شدند و پلاسميدهاي نو تركيب به نام DNA واكسنهاي pOMPH) و (pOMPA به دست آمدند. پنج گروه از جوجه (هر گروه شامل 20 قطعه جوجه 20n=) به صورت درون ماهيچهاي با دو پلاسميد نو تركيب، واكسن زنده تخفيف حدت يافته، كنترل ناقل pcDNA3.1(+) و PBS تزريق شدند. بعد از ايمنسازي، پاسخ ايمني و اثر محافظتي توسط سرولوژي و چالش ارزيابي شدند. افزايش چشمگير در ميزان آنتيبادي در سرم جوجههاي واكسينه شده با واكسن زنده تخفيف حدت يافته و همچنين دو DNA واكسن مشاهده گردد. به علاوه تست تكثير لنفوسيت (ميزان SI) در جوجههاي ايمنسازي شده با واكسن تخفيف حدت يافته و دو DNA واكسن بيشتر از جوجههاي تزريق شده با pcDNA3.1(+) و PBS بود (P<0.05)، از اين گذشته دو DNA واكسن محافظت محدود در جوجههاي واكسينه شده ايجاد كردند اگرچه اين محافظت كمتر از محافظت ايجاد شده توسط واكسن زنده تخفيف حدت يافته بود.
Abstract
Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodox vaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. In this study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into the eukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccines (pOMPH and pOMPA) were obtained. Five groups of chickens (n=20 per group) were intramuscularly injected with the two recombinant plasmids, attenuated live vaccine, control vector pcDNA3.1(+) and PBS, respectively. The immune responses and protective efficacy were evaluated after immunization by serological and challenging. A significant increase in serum antibody levels was observed in chickens vaccinated with the attenuated live vaccine and the two DNA vaccines. Additionally, the lymphocyte proliferation (SI values) were higher in chickens immunized with the attenuated live vaccine and the two DNA vaccines than in those vaccinated with pcDNA3.1(+) and PBS (P<0.05). Furthermore, the two DNA vaccines provided partial protection to the vaccinated chickens; however, the protective efficacy was inferior to that provided by the attenuated live vaccine.
Journal title
Iranian Journal of Veterinary Research (IJVR)
Serial Year
2013
Journal title
Iranian Journal of Veterinary Research (IJVR)
Record number
1595876
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