Isolation of Rhizobium and cost effective production of biofer tilizer

The aim of present study was the production of cost effective biofertilizer using optimized media for Rhizobium to meet increasing nutritional food requirements with biotechnology to increase crop yields by reducing use of chemical fertilizers to maintain ecological balance for sustainable production in minimum cost to increase percent of proteins, vitamins, nitrogen containing products which helps to increase yield, physical and chemical profile of soil to evaluate the fertility status has been observed during study that optimized inoculants with coal powder added is more beneficial when compare with laboratories activated charcoal powder for germination rate and growth of plants.After cultivation of experimental plants it is noted that 20-60 kg nitrogen remain back which benefits for further plantation of leguminous plants.