Author/Authors :
MOHAMMED، D.S S نويسنده Department of Microbiology, Faculty of Natural Sciences, Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria MOHAMMED, D.S S , Kumar، Shrestha Niranjan نويسنده , , DAMISA، D نويسنده Department of Microbiology, School of Science and Science Edu., Federal University of Technology, Minna, Niger State, Nigeria DAMISA, D , BALA، E نويسنده Department of Microbiology, Faculty of Natural Sciences, Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria BALA, E , MUHAMMAD، I L نويسنده Department of Microbiology, Faculty of Natural Sciences, Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria MUHAMMAD, I L , MUHAMMAD، G R نويسنده Department of Microbiology, Faculty of Natural Sciences, Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria MUHAMMAD, G R , YUNUSA، A نويسنده Department of Biological Sciences, Faculty of Natural Sciences, Ibrahim Badamasi Babangida Univ., Lapai, Niger State, Nigeria YUNUSA, A
Abstract :
This study was aimed for isolation, characterization and identification of fungi associated with decayed sweet oranges
collected from Lapai, Niger State, Nigeria. Samples of sweet oranges were collected from Police Barrack, Minna Motor Park,
Badeggi Market, Soje Garrage and Unguwan Gwari from Lapai. Segments (3-5 cm) of tissue from the margins of decayed C.
sinensis were cut with sterile scalpel and were placed on the prepared Sabouraud DextroseAgar in petri dishes and incubated at 28
± 1 C for 5 days. The mycelia of isolated fungi were fixed in lactophenol cotton blue on slides and were viewed under the
microscope. The fungi isolated were Fusarium oxysporum, Aspergillus niger, Candida tropicalis, Rhizopus stolonifer, Aspergillus
flavus, Fusarium solani, Penicillium chrysogenum and P. digitatum. A. niger was the predominant fungus isolated from all the
samples collected, while P. chrysogenum and P.digitatum were the least fungi isolated in this study. C. sinensis collected from Soje
Garrage had the highest occurrence of fungal isolates 8 (32%) followed by sweet oranges from Minna Motor Park with 5 (20%) of
fungal isolates, while sweet oranges from Police Barrack, Badeggi Market and Unguwan Gwari had the least occurrence of fungal
isolates 4 (16%) each. The pathogenicity tests showed that the fungal isolates isolated from the decayed sweet oranges were able to
produce the same signs in the healthy oranges when re-inoculated.
