Author/Authors :
Abnosi، M.H نويسنده Biology Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran , , Solemani Mehranjani، M نويسنده Biology Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran , , Momeni، H.R نويسنده Biology Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran , , Mahdiyeh Najafabadi، M نويسنده Biology Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran , , Barati، M نويسنده Biology Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran , , Shojafar، E نويسنده logy Dept., Faculty of Sciences, Arak University, Arak, 38156-8-8349, Markazi Provence, Iran ,
Abstract :
P-Nonylphenol (p-NP) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,
detergents and so on. Our previous study showed that the p-NP causes reduction in viability of the rats Bone
Marrow Mesenchymal Stem cells (MSCs). The aim of the present study was to investigate the mechanism of the
cell death due to p-NP exposure. The cells, after 3rd passage were treated with 100?M p-NP for a period of 36 hrs,
then using TUNEL, caspase and comet assay as well as fluorescent dye like Hochest, acridine orange and
monodansylcadaverin (MDC) staining the mechanism of cell death was studied. The results of this study showed
that the p-NP treated cells were TUNEL positive and also, activated caspase-3 enzyme in their cytoplasm was
visualized. Hochest staining showed chromatin condensation and nuclei breakage, at which the nuclear breakage
was further confirmed by comet assay. In addition, staining the cells with acridine orange revealed that the
cytoplasm of the treated cells contained numerous vesicles. Furthermore, staining of the cells with MDC showed
the presence of the double layer autophagic vacuoles. In conclusion, the induced cell death due to p-NP toxicity
was determined to be caspase dependent apoptosis as well as autophagy.
