Pr oduction and characterization of multiple drug r esistant cultur es isolated fr om Hospital Pr emises

Acurrent phenomenon of great concern among the medical communities in developing countries is raising multi-drug resistant organisms, and the challenges of curing the infections in human and animals. Five MDR cultures of bacteria were isolated from two different locations of Bikaner, Govt. P.B.M. Hospital & Kothari Hospital. Based upon Gram staining two isolates (S1C-V & S2SC-V) were identified as Gram negative which are Cocci and out off three Gram positive isolate (S2SC-II, S2SC-III, S2SC-IV) two were roads and one was cocci. Based upon the biochemical analysis S1,C-V bacterial culture was identified as Veillonella, for multidrug resistance(MDR)test eight different antibiotics in different concentration were used. It was observed that S1C-V (Veillonella ) bacterial culture showed resistance to Seven different antibiotics at different concentration from 10 micro g/ml to 100 micro g/ml. Bacterial culture S2SC-II (Bacillus) showed resistance to Six different antibiotics at 100 micro g/ml concentration. Bacterial culture S2SC-III (Sprorlactobacillus) showed resistance to five different antibiotics at 100 micro g/ml concentration. Bacterial culture S2SC-IV(Streptococcus) showed resistance to four different antibiotics at 100 micro g/ml concentration. Bacterial culture S2SC-V (Neisseria) showed resistance to seven different antibiotics at 10 micro g/ml to100 micro g/ml concentration.