Effect of red and near-infrared laser light on adenosine triphosphate (ATP) in the luciferine–luciferase reaction

Adenosine triphosphate (ATP) is an important molecule in biology because it stores chemical energy and releases it to the biochemical processes occurring in the cell. In this study we analysed the biochemical behaviour of ATP after irradiating it with 635 and 830 nm diode lasers. We analysed the luminescence peak, the reaction rate and the area under the luminescence curve at 2×10−9 mol/l of ATP in the luciferine–luciferase luminescence reaction before and after irradiating the molecule at several irradiances and radiant exposures. The absorption spectrum of ATP at 3×10−3 mol/l concentration was measured between 650 and 900 nm after laser irradiation at 635 nm (Argon-Dye) and 830 nm (diode laser). We found significant differences in the measured parameters when ATP was irradiated with both wavelengths. The absorption spectra of non-irradiated and irradiated ATP show a physical–chemical difference in the ATP molecule after irradiation with both lasers. We can conclude that visible and near-IR laser light with the parameters that were used in this study changed the biochemical behaviour of ATP molecules.