Efficient activation of carboxyl polysaccharides for the preparation of conjugates

We demonstrated here a highly efficient activation of carboxyl groups applicable to any polysaccharide. It was done by using the alternative activation agent, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. More specifically, we prepared glucans bearing linkers of different lengths that possessed masked carbonyl groups.