Inhibition effect on tobacco mosaic virus and regulation effect on calreticulin of oligochitosan in tobacco by induced Ca2+ influx

Oligochitosan induce plant resistance against plant virus via different signal transduction pathways. Here, the Ca2+ signaling pathway was investigated by which oligochitosan elicited defense responses to tobacco mosaic virus (TMV) in tobacco (Nicotiana tabacum cv. Huangmiaoyu nn) plants. The results showed that the multiplication and movement of TMV in tobacco plants were inhibited by oligochitosan. Oligochitosan also increased cytosolic free calcium ions and regulated the expression of calreticulin. Furthermore, the inhibitory effect on TMV and the regulation effect on calreticulin were depressed when blocking the Ca2+ signaling pathway by EGTA. These results indicated that oligochitosan induced tobacco resistance to TMV through Ca2+ signaling pathway.