An anticoagulant effect and chemical characterization of Lythrum salicaria L. glycoconjugates

An ion-exchange chromatography of Lythrum salicaria glycoconjugate afforded twelve fractions (LsF1–F12) by stepwise elution with water, sodium chloride and sodium hydroxide solutions. Carbohydrate, phenolic and protein moieties have been determined in all fractions. Individual glycoconjugates LsF1–F12 varied in carbohydrate, phenolic and protein contents, molecular mass and monosaccharide composition. Rhamnogalacturonans and/or homogalacturonans associated with arabinogalactans, arabinan and hexosan types of polysaccharides were identified in ion-exchange fractions. In vitro anticoagulant activity tests showed complete inhibition of plasma clot formation by LsF2, LsF7 and LsF12 fractions and a slight procoagulant effect of LsF3 and LsF4 ones while other ion-exchange fractions were not active.