Composition identification of Salvia extracts and testing of its inhibiting myocytes cell death caused by hypoxia/reoxygenation

A four-factor, three-level factorial design was used to evaluate the effects of the following factors on the extraction efficiency: extraction temperature (A), extraction time (B), particle size (C) and ratio of water to solid (D). The optimal conditions for extraction of Salvia heteroglycan were determined, using the ridge analysis, as extracting 2.5 h at 80 °C for three times. HPLC analyses showed the presence of rhamnose, glucose and galactose as constituents of Salvia heteroglycan. A water and ethanol extract of Salvia could enhance cell viability, Fas protein expression and myocardial apoptosis index in a dose-dependent manner. These indicated that water and ethanol extract of Salvia may reduce hypoxia/reoxygenation injury of myocytes cells.