Author/Authors :
Liu، نويسنده , , Jicheng and Sun، نويسنده , , Yongxu and Yu، نويسنده , , Haitao and Zhang، نويسنده , , Chunjing and Yue، نويسنده , , Liling and Yang، نويسنده , , Xiuzhen and Wang، نويسنده , , Liping and Liu، نويسنده , , Jianhua، نويسنده ,
Abstract :
One water-soluble glucan (PCP-W1) was purified from the crude polysaccharide of Pleurotus citrinopileatus by chromatography on DEAE Sepharose Fast Flow and Sephadex G-200 column, and PCP-W1 (Mw = 45 kDa), was predominantly composed of Glc. Partial acid hydrolysis, Smith degradation–periodate oxidation, methylation analysis, Fourier transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC–MS) analysis and nuclear magnetic resonance spectroscopy (NMR) experiments were conducted to elucidate its structure. The results indicated that PCP-W1 had a glucan backbone consisting of (1 → 6)-linked-β-d-glucopyranosyl residues, which were branched at O-3 position of the backbone with (1 → 3)-linked-β-d-glucopyranosyl and non-reducing end 1-β-d-glucopyranosyl residues. The repeating unit of the polysaccharide was established as:
