Title of article
Structure elucidation of an immunoenhancing pectic polysaccharide isolated from aqueous extract of pods of green bean (Phaseolus vulgaris L.)
Author/Authors
Patra، نويسنده , , Pradip and Das، نويسنده , , Debsankar and Behera، نويسنده , , Birendra and Maiti، نويسنده , , Tapas K. and Islam، نويسنده , , Syed S.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2012
Pages
7
From page
2169
To page
2175
Abstract
A water-soluble pectic polysaccharide (PS-I) isolated from the hot water extract of the pods of green bean (Phaseolus vulgaris L.) by successive purification through dialysis, centrifugation and Sepharose-6B gel filtration was found to consist of methyl ester of d-galacturonic acid, d-galactose, and l-arabinose in a molar ratio of nearly 2:2:1. This molecule showed splenocyte, thymocyte activation as well as antioxidant properties. Structural studies of PS-I was performed using acid hydrolysis, methylation analysis, periodate oxidation studies along with NMR analysis (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC) and the repeating unit of PS-I was established as:
Keywords
NMR spectroscopy , structure , Immunoenhancing activity , antioxidant activity , pectin , Phaseolus vulgaris L.
Journal title
CARBOHYDRATE POLYMERS
Serial Year
2012
Journal title
CARBOHYDRATE POLYMERS
Record number
1623348
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