Title of article
Expression patterns of chitinase produced from Paenibacillus chitinolyticus with different two culture media
Author/Authors
Song، نويسنده , , Yong-Su and Seo، نويسنده , , Dong-Jun and Kim، نويسنده , , Kil-Yong and Park، نويسنده , , Ro-Dong and Jung، نويسنده , , Woo-Jin، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2012
Pages
6
From page
1187
To page
1192
Abstract
To investigate the expression patterns of chitinase isozymes on native-PAGE and SDS-PAGE gels Paenibacillus chitinolyticus MP-306 was cultured on culture media with and without chitin substrate. P. chitinolyticus MP-306 had a strong chitinolytic activity on colloidal chitin medium. Chitinase isozymes of MP-306 were expressed as six bands (CN1–CN6) on native-PAGE gels and thirteen bands (CS1–CS13) on SDS-PAGE gels after incubation in chitin medium. Three bands (CN1, CN2, and CN3) of chitinase isozymes of MP-306 on native-PAGE gels were expressed as nine bands (CS1, CS2, CS3, CS4, CS5, CS6, CS8, CS10, and CS13) of chitinase isozymes on SDS-PAGE gels. Three bands (CN4, CN5, and CN6) of chitinase isozymes of MP-306 were strongly inhibited by metal ions on native-PAGE and SDS-PAGE gels.
Keywords
Colloidal chitin , Chitinase isozymes , Paenibacillus chitinolyticus
Journal title
CARBOHYDRATE POLYMERS
Serial Year
2012
Journal title
CARBOHYDRATE POLYMERS
Record number
1623973
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