In vitro antiviral activity of sulfated Auricularia auricula polysaccharides

Total Auricularia auricula polysaccharide (AAPt) was prepared by extracting and removing the proteins. Column chromatography was used to further graded it into AAP1 and AAP2. Three AAPs were modified by chlorosulfonic acid–pyridine method to obtain three sulfated AAPs (sAAPs), sAAPt, sAAP1 and sAAP2, respectively. Three sAAPs and Newcastle disease virus (NDV) were added into cultivation system of chicken embryo fibroblast (CEF) in three manners, pre-, post- and simultaneous-adding polysaccharide with NDV respectively, taking three non-modified AAPs as control. Their anti-viral activities were compared by MTT method. The results showed that sAAPs and AAPs at a certain concentration could significantly inhibit the cellular infectivity of NDV in three manners. The effects of sAAPs were better than that of AAPs. It indicated that sulfated modification could enhance the antiviral activity of AAP. sAAP1 and sAAPt possessed stronger activity and would be as the component of a new-type antiviral drug.