Title of article :
In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion
Author/Authors :
Hansson، نويسنده , , Annasara and Hashom، نويسنده , , Nour and Falson، نويسنده , , Françoise and Rousselle، نويسنده , , Patricia J. Jordan، نويسنده , , Olivier and Borchard، نويسنده , , Gerrit، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration of 59 nmol/mg polymer to increase cell–biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3–5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall, CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing.
Keywords :
Wound healing , Human dermal fibroblast , Chitosan , RGD peptide , cell adhesion
Journal title :
CARBOHYDRATE POLYMERS
Journal title :
CARBOHYDRATE POLYMERS