Author/Authors :
Zhu، نويسنده , , Zhen-yuan and Liu، نويسنده , , Yang and Si، نويسنده , , Chuan-Ling and Yuan، نويسنده , , Jing-Hua Lv، نويسنده , , Qiang and Li، نويسنده , , Yuan-Yuan and Dong، نويسنده , , Guo-Ling and Liu، نويسنده , , An-Jun and Zhang، نويسنده , , Yong-Min، نويسنده ,
Abstract :
A chemically new sulfated polysaccharide (SPS50) was prepared from the water soluble polysaccharide (PS50), isolated from Cordyceps_gunnii mycelia, by concentrated sulfuric acid method. The yield of crude SPS50 was 62.34% and its specific rotation was [α]D20 = −36.75°. The structural characteristics of this chemically sulfated polysaccharide were determined based on the infrared analysis (IR), high performance liquid chromatography (HPLC) and sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE). Its biological properties including anti-oxidant and anti-tumor activities were also investigated. The results showed that the anti-oxidant capacity of SPS50 was not as good as SP50 and the anti-tumor activity of SPS50 was much better than PS50. SPS50 showed evident growth inhibition on K562 cells. The tumor inhibition ratio of SPS50 against K562 cells was 69.92%.
Keywords :
Sulfated modification , polysaccharide , Anti-oxidant Activity , Anti-tumor activity , Cordyceps gunnii
