Ethylenediaminediacetic acid bis(carbido amide chitosan): Synthesis and evaluation as solid carrier to fabricate nanoemulsion

We have optimized the synthesis of spray dried ethylenediaminediacetic acid bis(carbido amide chitosan) (ED-chitosan) microparticles employing 32 full factorial design. The ED-chitosan microparticles were characterized using FTIR-ATR, DSC analysis, oil adsorbing capacity, oil desorbing capacity, surface free energy and dynamic advancing contact angle. The ED-chitosan microparticles were found to have enhanced dispersive component of surface free energy as compared to Aerosil 200. However, they showed lower polar component. This was associated with similar oil adsorbing capacity but higher desorbing capacity. The SEM analysis supported ability of ED-chitosan microparticles to completely spread lipophillic drug over its surface. The reconstituted nanoemulsion generated from ED-chitosan was stable (neutral zeta potential), spherical shaped and 110 nm size (TEM analysis). Further, these nanoemulsions when prepared with primaquine were successfully enhanced in vitro dissolution and its ex vivo performance. Thus, the study showed new possibilities of industrial acceptance of ED-chitosan microparticles as a solid carrier for the fabrication of nanoemulsion.