Title of article
Glucogalactan: A polysaccharide isolated from the cell-wall of Verticillium Lecanii
Author/Authors
Corradi da Silva، نويسنده , , Maria L. and Exposti، نويسنده , , Diego T.D. and Vasconcelos، نويسنده , , Ana F.D. and Alexandre، نويسنده , , Samara M.A. and Silveira، نويسنده , , Joana L.M. and Ducatti، نويسنده , , Diogo R.B. Ducatti، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
7
From page
1353
To page
1359
Abstract
A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D 1H and 13C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established:
n ≈ 22 and m ≈ 22.
Keywords
verticillium Lecanii , Lecanicillium lecanii , Glucogalactan , NMR spectroscopy
Journal title
CARBOHYDRATE POLYMERS
Serial Year
2013
Journal title
CARBOHYDRATE POLYMERS
Record number
1625164
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