Preparation and detailed NMR analyses of a series of oligo-α-carrageenans

Standard oligo-ι-carrageenans were produced with ι-carrageenases isolated from the marine bacteria Alteromonas fortis and Pseudolalteromonas atlantica. The carrageenans were then desulfated using a 4S-carrageenan-sulfatase purified from P. atlantica. Using chromatography and NMR analyses, we characterized a series of new standard neo-α-carrageenan oligosaccharides.