Author/Authors :
Gambini، نويسنده , , Juan and Gomez-Cabrera، نويسنده , , Mari Carmen and Borras، نويسنده , , Consuelo and Valles، نويسنده , , Soraya L. and Lopez-Grueso، نويسنده , , Raul and Martinez-Bello، نويسنده , , Vladimir E. and Herranz، نويسنده , , Daniel and Pallardo، نويسنده , , Federico V. and Tresguerres، نويسنده , , Jesus A.F. and Serrano، نويسنده , , Manuel and Viٌa، نويسنده , , Jose، نويسنده ,
Abstract :
Sirtuins are deacetylases involved in metabolic regulation and longevity. Our aim was to test the hypothesis that they are subjected to redox regulation by the [NADH]/[NAD+] ratio. We used NIH3T3 fibroblasts in culture, Drosophila fed with or without ethanol and exercising rats. In all three models an increase in [NADH]/[NAD+] came up with an increased expression of sirtuin mRNA and protein. PGC-1α (a substrate of sirtuins) protein level was significantly increased in fibroblasts incubated with lactate and pyruvate but this effect was lost in fibroblasts obtained from sirtuin-deficient mice.
clude that the expression of sirtuins is subject to tight redox regulation by the [NADH]/[NAD+] ratio, which is a major sensor for metabolite availability conserved from invertebrates to vertebrates.
Keywords :
Pyruvate , lactate , Drosophila , Rat , Ethanol , Exercise
