Author/Authors :
Alves، نويسنده , , Jackson Nogueira and Pires، نويسنده , , Karla Maria Pereira and Lanzetti، نويسنده , , Manuella and Barroso، نويسنده , , Marina Valente and Benjamim، نويسنده , , Clلudia Farias and Costa، نويسنده , , Cristiane Aguiar and Resende، نويسنده , , Angela Castro and Santos، نويسنده , , Juliana Carvalho and Ribeiro، نويسنده , , Marcelo Lima and Porto، نويسنده , , Luيs Cristَvمo and Valença، نويسنده , , Samuel Santos Valença، نويسنده ,
Abstract :
Our aim was to investigate CCR2 and HMGB1 involvement in a murine model of endotoxic shock. We used C57BL/6 CCR2 knockout (KO) mice and wild-type (WT) littermates to establish an optimal dose of LPS. CCR2 KO mice survived more frequently than WT mice after 80, 40 and 20 mg/kg of LPS i.p. Inflammation and redox markers were high in WT mice than in CCR2 KO mice. HMGB1 expression was reduced in CCR2 KO mice in parallel to ERK 1/2 activation. Therefore, we used glycyrrhizic acid (50 mg/kg), an HMGB1 inhibitor in WT mice injected with LPS, and mortality was fully abolished. Thus, drugs targeting CCR2 and HMGB1 could represent future resources for sepsis treatment.
Keywords :
Sepsis , HMGB1 , CCR2 , MCP-1 , Glycyrrhizic acid
