Title of article :
A Clark-type oxygen chip for in situ estimation of the respiratory activity of adhering cells
Author/Authors :
Wu، نويسنده , , Ching-Chou and Luk، نويسنده , , Hsiang-Ning and Lin، نويسنده , , Yen-Ting Tsai and Yuan، نويسنده , , Chia-Yin، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2010
Pages :
7
From page :
228
To page :
234
Abstract :
A Clark-type oxygen chip consisting of a polydimethylsiloxane (PDMS) reservoir containing an amino group-modified PDMS oxygen-permeable membrane (OPM) and a glass substrate containing a three-electrode detector has been constructed by using microfabrication techniques, and it is utilized for in situ measurement of the respiration activity of adhering cells. Use of the alginate sol electrolyte and the electroplating Ag/AgCl pseudo-reference electrode can effectively diminish the crosstalk between the electrochemical electrodes and supply a stable potential for the detection of dissolved oxygen, respectively. The Clark-type oxygen chips possess only 1.00% residual current, response time of 13.4 s and good linearity with a correlation coefficient of 0.9933. The modification of amino groups for the OPM obviously facilitates the adhesion of HeLa cells onto the PDMS OPM surface and allows the cells to spread after 2 h of incubation. The oxygen consumption of the cells in the cell-adhesion process increases with the adhesion time, and the increment of cellular oxygen consumption per minute reaches a maximum after 30 min of incubation. Moreover, the change in the respiration activity of adhering HeLa cells stimulated by the high concentration of glucose or propofol anaesthetic can be monitored in real time with the Clark-type oxygen chip.
Keywords :
Sol electrolyte , Oxygen consumption , Respiration activity , Clark-type , Oxygen chip
Journal title :
Talanta
Serial Year :
2010
Journal title :
Talanta
Record number :
1636899
Link To Document :
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