Title of article
Design, synthesis, and characterization of a calcium-sensitive near infrared dye
Author/Authors
Ellis، نويسنده , , April L. and Christian Mason، نويسنده , , J. and Lee، نويسنده , , Hsiau-Wei and Strekowski، نويسنده , , Lucjan and Patonay، نويسنده , , Gabor and Choi، نويسنده , , Hoseob and Yang، نويسنده , , Jenny J.، نويسنده ,
Issue Information
ماهنامه با شماره پیاپی سال 2002
Pages
9
From page
1099
To page
1107
Abstract
Intracellular calcium concentration in biological cells varies from 0.1 to 10 μM depending upon cell signaling and disease states. A direct estimate of calcium concentration in cell tissues within this range is possible with a novel calcium-selective reagent 15C5-774. The molecule of 15C5-774 consists of a near-infrared (NIR) chromophore (λmax=774 nm) and a metal complexing moiety of benzo-15-crown-5. The reagent shows a strong calcium binding affinity in a 1:1 ratio and metal selectivity in the order Ca2+>Mg2+>Sr2+≈K+≈Na+>Zn2+>Li+. The high sensitivity is achieved by conducting absorption measurements in the NIR region where background interference from the biological matrix is low.
Keywords
calcium binding , Near infrared , Benzo-15-crown-5 , cyanine dye
Journal title
Talanta
Serial Year
2002
Journal title
Talanta
Record number
1642404
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