Author/Authors :
Hojo، نويسنده , , Hironobu and Watabe، نويسنده , , Jun and Nakahara، نويسنده , , Yoshiaki and Nakahara، نويسنده , , Yuko and Ito، نويسنده , , Yukishige and Nabeshima، نويسنده , , Kazuki and Toole، نويسنده , , Bryan P، نويسنده ,
Abstract :
The extracellular Ig domain I of Emmprin (34-94) carrying a chitobiose unit at Asn44 was chemically synthesized. Boc-Asn with a benzyl-protected chitobiose unit was synthesized and used for the preparation of peptide thioester with the sequence of Emmprin (34-58) by Boc strategy. C-Terminal peptide amide (59-94) was also prepared by the solid-phase method. These segments were condensed by activation of the thioester group by silver ions to obtain a protected form of Emmprin (34-94)-NH2. After deprotection and air oxidation, the desired Emmprin (34-94)-NH2 with chitobiose was successfully obtained.
