Study and analytical application of inhibitory effect of captopril on multienzyme redox system

Multienzyme redox system is the most important biological oxidation process in cellular respiration chain. Nicotinamide adanine dinucleotide redox state (NADH/NAD) can be directly control site in numerous biological processes linked to rhythm, senescence, cancer and death. Therefore, it is very important to investigate the influencing factor of this multienzyme redox system. We have already found a strong inhibitory effect of captopril on this enzyme-catalyzed reaction and further experimental results revealed that this inhibitory action belonged to competitive type. Based on this phenomenon, we developed a highly sensitive spectroflourimetry method for the determination of captopril in pharmaceuticals.