Author/Authors :
Wilk، نويسنده , , Andrzej G. Chmielewski، نويسنده , , Marcin K and Grajkowski، نويسنده , , Andrzej and Phillips، نويسنده , , Lawrence R and Beaucage، نويسنده , , Serge L، نويسنده ,
Abstract :
An efficient and economical method for the solid-phase synthesis of oligodeoxyribonucleotides and their phosphorothioate analogues is described. The method entails the use of the 4-oxopentyl group for phosphate/thiophosphate protection. Post-synthesis removal of the protecting group is easily and rapidly achieved under mild conditions at ambient temperature using either pressurized gaseous amines or concentrated ammonium hydroxide.
