Author/Authors :
Esteves da Silva، نويسنده , , Joaquim C.G. and Magalhمes، نويسنده , , Jْlia M.C.S. and Fontes، نويسنده , , Rui، نويسنده ,
Abstract :
Firefly oxyluciferin (2-(6′-hydroxybenzothiazolyl)-4-hydroxythiazole) was chemically synthesized and characterized by means of 13C and 1H NMR, UV–vis spectrometry and RP-HPLC using different pH elution conditions. One of the chromatographic peaks observed in luciferase-catalyzed reaction mixtures was identified as corresponding to oxyluciferin.
