Modeling and simulation of clean fuel production by Enterobacter cloacae IIT-BT 08

Cell growth and substrate degradation kinetics of newly isolated Enterobacter cloacae IIT-BT 08 were investigated with the help of Monod model. The values of μmax, KS and YX/S of the cell were 0.568 h−1, 3.658 g l−1 and 0.0837, respectively, using glucose as a substrate. The simulated profiles of the substrate concentration and cell mass concentration had significant variance with respect to the experimental values. This might be due to the substrate inhibition as the product is a gas. A modified Andrew’s model for substrate inhibition was suggested and found to have good resemblance with the experimental results. In other studies, hydrogen production was found to be growth associated.