Title of article :
FTIR-determination of sterols from the red alga Asparagopsis armata: Comparative studies with HPLC
Author/Authors :
El Hattab، نويسنده , , Naïma and Daghbouche، نويسنده , , Yasmina and Hattab، نويسنده , , Mohamed El and Piovetti، نويسنده , , Louis and Garrigues، نويسنده , , Salvador and Guardia، نويسنده , , Miguel de la، نويسنده ,
Issue Information :
ماهنامه با شماره پیاپی سال 2006
Abstract :
An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase cartridges (SPE) and eluted with dichloromethane stabilized with β-amylene. Fourier transform infrared (FTIR) spectrometry was employed for selective detection at 1049 cm−1 with a baseline established between 1000 and 1079 cm−1. The results were compared to those obtained by high performance liquid chromatography (HPLC). The concentration obtained in actual samples from alga was 3.37% (w/w) by FTIR and 3.30% (w/w) by HPLC, showing a good comparability between the two methods.
Keywords :
Sterol determination , High Performance Liquid Chromatography , red alga , Fourier transform infrared , Solid phase extraction , Asparagopsis armata
