Freeing anomeric hydroxyl groups of peracetylated oligosaccharides by Aspergillus niger lipase

Various peracetylated oligosaccharides (di- to hepta-) are subjected to regioselective hydrolysis by Aspergillus niger lipase hydrolyzing 1-O-acetyl group. Molecular weight, anomeric and interglycosidic linkage configurations play a significant role in the hydrolysis reaction. Useful 1-O-deprotected material can be easily prepared for further chemical elaboration.