Author/Authors :
Shibuya، نويسنده , , Masaaki and Nishioka، نويسنده , , Mizue and Sankawa، نويسنده , , Ushio and Ebizuka، نويسنده , , Yutaka، نويسنده ,
Abstract :
To investigate the mechanism of stilbene synthase (STS) reaction, the origin of the aromatic protons of resveratrol B-ring was examined using STS from Arachis hypogaea expressed in Escherichia coli and deuterated malonyl CoA. The presence of resveratrol labeled with three deuterium atoms was detected by GCMS analysis indicating decarboxylation earlier than aromatization, i.e. exclusion of intermediacy of stilbenecarboxylic acid in STS reaction.
