Liquid-phase microextraction and GC for the determination of primary, secondary and tertiary aromatic amines as their iodo-derivatives

Presence of iodine in aromatic amines, introduced by their reaction with iodine, and other electron withdrawing substituents such as chlorine and nitro, has been found to afford excellent liquid-phase microextraction (LPME) in toluene and separation by gas chromatography in the determination of primary, secondary and tertiary aromatic amines. The effect is due to decreased basic nature of amines when electronegative substituents are present. Single drop microextraction (SDME) of the amines in 2 μl of toluene and injection of the whole extract into GC, or LPME into 50 μl of toluene and injection of 2 μl of extract, were used. LPME has been found more robust and to give better extraction in shorter period than SDME. In SDME–GC–FID, the average correlation coefficient was 0.9939 and average limit of detection 25 μg l−1 (range 12–61 μg l−1) whereas the corresponding values in LPME–GC–MS were, respectively, 0.9953 and 33 ng l−1 (range 18–60 ng l−1). The method has been applied to determine aromatic amines in river water, dye factory effluents and food dye stuffs. The LPME was found as robust, rugged and simple extraction method.