Author/Authors :
Nishihama، نويسنده , , Syouhei and Imabayashi، نويسنده , , Hisano and Matoba، نويسنده , , Tomoko and Toya، نويسنده , , Chika and Watanabe، نويسنده , , Kosuke and Yoshizuka، نويسنده , , Kazuharu، نويسنده ,
Abstract :
A urinary protein assay has been investigated, employing a micro-flow injection analysis (μFIA) combined with an adsorptive separation of protein from analyte. The adsorptive separation part of protein in the artificial urine with ceramic hydroxyapatite is integrated on the μFIA chip, since the interference of other components coexisting in urine occurs in the conventional FIA system. The typical FI peak can be obtained following the adsorption–elution process of the protein prior to the detection, and the protein concentration in artificial urine can be quantitatively determined.