Detection of clindamycin by capillary electrophoresis with an end-column electrochemiluminescence of tris(2,2′-bypyridine)ruthenium(II)

Coupled capillary electrophoresis (CE) with end-column electrogenerated chemiluminescence (ECL) was adopted for the quantitative detection of clindamycin. Clindamycin enhanced ECL intensity of tris(2,2′-bypyridine)ruthenium(II) (Ru(bpy)32+) as a coreactant. Under the optimized conditions, the ECL intensity was linear with the concentration of clindamycin over the range from 5.0 × 10−7 to 1.0 × 10−4 M with a detection limit of 1.4 × 10−7 M. The proposed CE–ECL was successfully applied for the detection of clindamycin in pharmaceutical and clinic samples. The interaction of clindamycin with hemoglobin was also investigated. The binding constant of clindamycin with hemoglobin was estimated to be 3.6 × 103 M−1.