Author/Authors :
Shan، نويسنده , , Yaming and Wang، نويسنده , , Liping and Shi، نويسنده , , Yuhua and Zhang، نويسنده , , Hao and Li، نويسنده , , Hongmei and Liu، نويسنده , , Hanzhi and Yang، نويسنده , , Bai and Li، نويسنده , , Tianyu and Fang، نويسنده , , Xuexun and Li، نويسنده , , Wei، نويسنده ,
Abstract :
3-Mercaptopropyl acid-stabilized CdTe nanoparticles synthesized in aqueous solution are found to be able to conjugate with peptides or proteins mediated by N-hydroxysulfo-succinimide (NHS) but 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC). The reaction time and pH have been optimized. Gel-permeation HPLC was applied following the conjugation, which could quickly and simultaneously detect and purify the quantum dots (QDs) conjugates. The biological activities of QDs conjugates are maintained and give superior results in cell labeling. These results are encouraging regarding the application of QDs molecules for use in living cells, diagnostics and drug delivery.
Keywords :
NHS-mediated conjugation , Cell labeling , Nuclear targeting signal , Quantum dot
