A method to determine quercetin by enhanced luminol electrogenerated chemiluminescence (ECL) and quercetin autoxidation

Quercetin greatly enhanced luminol electrochemiluminescence of quercetin in alkaline solution. When the concentration of luminol was 0.1 mol L−1, the detection limit for quercetin was 2.0 × 10−8 mol L−1 with a linear range from 1.0 × 10−7 to 2 × 10−5 mol L−1. The pH and buffer substantially affected ECL intensity. Quercetin was autoxidized in alkaline aqueous solution. The rate of autoxidation of quercetin in various pH buffers and borate concentrations were measured. Borate was found to inhibit quercetin autoxidation and compromise quercetin enhancement effect on luminol ECL to some extent. Two final autoxidation products were identified with LC–MS methods. Autoxidation process was associated with enhancement of ECL intensity. The ROS generated during quercetin autoxidation enhanced the ECL intensity.