Validation of a new method using the reactivity of electrogenerated superoxide radical in the antioxidant capacity determination of flavonoids

This article lays out a new method to measure the antioxidant capacity of some flavonoids. The methodology developed is based on the kinetics of the reaction of the antioxidant substrate with the superoxide radical (O2−). A cyclic voltammetric technique was used to generate O2− by reduction of molecular oxygen in aprotic media. In the same experiment the consumption of the radical was directly measured by the anodic current decay of the superoxide radical oxidation in the presence of increasing concentrations of antioxidant substrate. The method was statistically validated on flavonoid monomers and on the standard antioxidants: trolox, ascorbic acid and phloroglucinol. The linear correlations between the anodic current of O2− and the substrate concentration allowed the determination of antioxidant index values expressed by the substrate concentration needed to consume 30% (AI30) and 50% (AI50) of O2− in given conditions of oxygen concentration and scanning rate. The fidelity of the method was examined intraday and interlaboratories.