Direct determination of uranium in seawater by laser fluorimetry

A method for estimation of uranium in seawater by using steady state laser flourimetry is described. Uranium present in seawater, in concentration of approximately 3 ng ml−1 was estimated without prior separation of matrix. Quenching effect of major ions (Cl−, Na+, SO4−, Mg+, Ca+, K+, HCO3−, Br−) present in seawater on fluorescence intensity of uranium was studied. The concentration of phosphoric acid required for maximum enhancement of fluorescence intensity was optimized and was found to be 5%. Similarly the volume of concentrated nitric acid required to eliminate the quenching effect of chloride and bromide completely from 5 ml of seawater were optimized and was found to be 3 ml. A simple equation was derived using steady state fluorescence correction method and was used for calculation of uranium concentration in seawater samples. The method has a precesion of 1% (1 s, n = 3). The values obtained from laser fluorimetry were validated by analyzing the same samples by linear sweep adsorptive stripping voltametry (LSASV) of the uranium–chloranilic acid (2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone) complex. Both the values are well in agreement.