Author/Authors :
Berry، نويسنده , , David A. and Jung، نويسنده , , Kee-Yong and Wise، نويسنده , , Dean S. and Sercel، نويسنده , , Anthony D. and Pearson، نويسنده , , William H. and Mackie، نويسنده , , Hugh and Randolph، نويسنده , , John B. and Somers، نويسنده , , Robert L.، نويسنده ,
Abstract :
Pyrrolo-dC (1a, 6-methyl-3-(2-deoxy-β-d-ribofuranosyl)-3H-pyrrolo[2,3-d]pyrimidin-2-one) and its cyanoethyl phosphoramidite 2a were synthesized. The latter was incorporated into oligodeoxyribonucleotides by standard automated synthesis techniques, where pyrrolo-dC was found to serve as a fluorescent analog of deoxycytidine. The cyanoethylphosphoramidite (2b) of pyrrolo-C (2a, 6-methyl-3-(β-d-ribofuranosyl)-3H-pyrrolo[2,3-d]pyrimidin-2-one) was also synthesized and may find use for the site-specific incorporation of a fluorescent cytidine analog into oligoribonucleotides.
