Author/Authors :
Lien، نويسنده , , Truong Thi Ngoc and Lam، نويسنده , , Tran Dai Nghiep، نويسنده , , Vu Thi Hong and Hoang، نويسنده , , Tran Vinh and Quang، نويسنده , , Duong Tuan and Khieu، نويسنده , , Dinh Quang and Tsukahara، نويسنده , , Toshifumi and Lee، نويسنده , , Young-Hoon and Kim، نويسنده , , Jong Seung، نويسنده ,
Abstract :
In this paper, we describe DNA electrochemical detection for genetically modified organism (GMO) based on multi-wall carbon nanotubes (MWCNTs)-doped polypyrrole (PPy). DNA hybridization is studied by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). An increase in DNA complementary target concentration results in a decrease in the faradic charge transfer resistance (Rct) and signifying “signal-on” behavior of MWCNTs-PPy-DNA system. QCM and EIS data indicated that the electroanalytical MWCNTs-PPy films were highly sensitive (as low as 4 pM of target can be detected with QCM technique). In principle, this system can be suitable not only for DNA but also for protein biosensor construction.
Keywords :
MWCNTs-doped polypyrrole , Electrochemical impedance spectroscopy , Electrochemical DNA biosensor , Quartz crystal microbalance
