A novel molecularly imprinted solid-phase microextraction fiber coupled with high performance liquid chromatography for analysis of trace estrogens in fishery samples

The combination of molecular imprinting and solid-phase microextraction (SPME) technique provides a powerful sample preparation tool in terms of selectivity, simplicity, and flexibility. This paper reports a novel molecularly imprinted polymer (MIP) coated SPME fiber with 17β-estradiol as template by improved multiple co-polymerization method. The obtained fiber exhibits excellent characteristics such as high porosity, good thermal and chemical stability. Extraction performance shows that the MIP-coated fiber has stronger affinity to the template molecule as compared with the commercial SPME fibers and the control polymer-coated fiber without addition of template. Owing to the shape and structural compatibility, the obtained fiber also demonstrated specific selectivity to the structural related compounds of 17β-estradiol, such as estriol, estrone and 17α-ethynylestradiol, and thus can be applied to simultaneous determination of these estrogens from complex samples coupled with high performance liquid chromatography. The variables that influence extraction were investigated. The MIP-coated fiber demonstrated its efficiency for extraction of estrogens in fishery samples. The detection limits were in the range of 0.98–2.39 μg L−1, and the recoveries were 80.0–83.6% and 85.0–94.1% for fish and shrimp tissue samples, respectively.