Title of article
A fluorogenic assay for transketolase from Saccharomyces cerevisiae
Author/Authors
Sevestre، نويسنده , , Aurélie and Hélaine، نويسنده , , Virgil and Guyot، نويسنده , , Ghislain and Martin، نويسنده , , Christine and Hecquet، نويسنده , , Laurence، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2003
Pages
4
From page
827
To page
830
Abstract
In order to generate transketolase (TK) with new or improved properties by in vitro evolution, an efficient screening system is an absolute prerequisite for identifying the evolved enzyme variants. We report here an assay allowing us to detect wild type TK activity in vitro by fluorescence. We examined the use of the fluorogenic compound 1 as donor substrate of TK, which is itself non fluorescent but releases a fluorescent product: umbelliferone.
Journal title
Tetrahedron Letters
Serial Year
2003
Journal title
Tetrahedron Letters
Record number
1660313
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