Author/Authors :
Pramanik، نويسنده , , Birendra N. and Ing، نويسنده , , Yao Hain and Bose، نويسنده , , Ajay K. and Zhang، نويسنده , , Li-Kang and Liu، نويسنده , , Yan-Hui and Ganguly، نويسنده , , Subhendu N. and Bartner، نويسنده , , Peter، نويسنده ,
Abstract :
The application of microwave assisted modification of the Akabori hydrazinolysis reaction has been found to cleave cyclic oligopeptides in a selective fashion to produce mainly the hydrazide of a specific linear peptide. This ring cleavage requires a few minutes of reaction in a domestic microwave oven. The linear peptide hydrazide can be analyzed by ESI-MS/MS, FAB-MS, and FAB-MS/MS methods for the determination of the amino acid sequence.
Keywords :
Akabori reaction , Hydrazinolysis , cyclopeptides , amino acid sequence , microwave enhanced reaction
