Inkjet-printed polyaniline patterns for exocytosed molecule detection from live cells

Polyaniline (PANi) patterns on flexible substrate are fabricated for biomolecule detection from live cells. PANi patterns are prepared by inkjet printing on polyethylene terephthalate film. Subsequently, arginine–glycine–aspartate (RGD) peptide is immobilized on the PANi pattern to selectively adhere cells. Rat pheochromocytoma PC12 cells are cultured on the RGD–immobilized PANi pattern, and patterned with high selectivity and growth. Additionally, the cells show focal adhesion on the RGD–immobilized PANi pattern, which are confirmed with vinculin staining and scanning electron microscopic images. To monitor dynamic biomolecular release from PC12 cells, RGD–immobilized PANi pattern is used for a real-time electrical signal detector. RGD–immobilized PANi patterning and sensing system represents outstanding ability to translate and amplify exocytosis molecules into a detectable signal as a transducer.