Author/Authors :
Kurz?tkowska، نويسنده , , Katarzyna and Mielecki، نويسنده , , Marcin and Grzelak، نويسنده , , Krystyna and Verwilst، نويسنده , , Peter and Dehaen، نويسنده , , Wim and Radecki، نويسنده , , Jerzy and Radecka، نويسنده , , Hanna، نويسنده ,
Abstract :
New surface plasmon resonance (SPR) sensing platforms which consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His6-JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His6-JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.
Keywords :
His-tagged protein immobilization , Surface plasmon resonance biosensors , Janus kinase 2 , Pentetic acid thiol ligand-Cu(II) , Kinetic and thermodynamic analysis , Dipyrromethene-Cu(II)
